The image depicts spores in potassium hydroxide (KOH) of a white rust fungus species from a squaw-weed plant. The spores are circular and appear to be yellowish with gray centers. They are not uniform in size or shape.
In general, KOH is used as an agent to clear away the cell walls of fungi so that they can be easily observed under a microscope. If you were to use this image to identify another specimen, it would likely require several steps:
1. Collect and prepare the sample: Take a small portion of the fungus in question and place it on a slide.
2. Apply KOH to the slide: Use a dropper to add one or two drops of KOH solution to the center of the fungal material on the slide.
3. Observe the specimen under a microscope: Once the sample has been prepared, use a microscope with 40x, 100x, and 400x objectives to examine its morphology.
**Important note:** The identification of a fungus can be challenging and requires expertise in mycology (the study of fungi). It is highly recommended that you consult an expert or a reliable field guide before attempting to identify a specimen. – AI vision
Las esporas (en KOH) del hongo de la roya blanca Pustula tragopogonis (Pustula obtusata, Albugo tragopogonis) (?) de una hoja de hierba gatera (Senecio ampullaceus), recolectada cerca de Birch Creek Unit of Somerville Lake State Park. Texas, 5 de marzo de 2023. La imagen muestra las esporas en hidróxido potásico (KOH) de una especie de hongo de la roya blanca de una planta de hierba gatera. Las esporas son circulares y parecen ser amarillentas con centros grises. No tienen forma ni tamaño uniformes. En general, el KOH se utiliza como agente para eliminar las paredes celulares de los hongos para que puedan observarse fácilmente bajo un microscopio. Si se utiliza esta imagen para identificar otra muestra, sería probable que se requieran varias etapas:
1. Recolectar y preparar la muestra: tomar una pequeña porción del hongo en cuestión y colocarlo en un portaobjetos. 2. Aplicar KOH al portaobjetos: use un cuentagotas para agregar una o dos gotas de solución de KOH al centro del material fúngico en el portaobjetos. 3. Observar la muestra bajo un microscopio: una vez que se haya preparado la muestra, use un microscopio con objetivos de 40x, 100x y 400x para examinar su morfología. **Importante**: La identificación de un hongo puede ser un desafío y requiere experiencia en micología (el estudio de los hongos). Se recomienda encarecidamente consultar a un experto o una guía de campo confiable antes de intentar identificar una muestra. – Spanish translation
Similar(1): Leaf of squaw-weed (Senecio ampullaceus) with sunflower white rust ... Somerville Lake State Park. Texas, March 5, 2023
Similar(2): Underside of a leaf of squaw-weed (Senecio ampullaceus) with sunflower ... Somerville Lake State Park. Texas, March 5, 2023
Similar(3): Spores (in Melzer) of sunflower white rust fungus Pustula tragopogonis ... on-the-Brazos a day before. Texas, March 7, 2023
Similar(4): Spores an conidia(?) (in KOH) of sunflower white rust fungus Pustula ... on-the-Brazos a day before. Texas, March 7, 2023
Similar(5): Underside of a leaf of squaw-weed (Senecio ampullaceus) with sunflower ... Historic Site. Washington, Texas, March 6, 2023
Similar(6): Underside of leaves of squaw-weed (Senecio ampullaceus) with sunflower ... Creek Park. College Station, Texas, March 20, 2023
Similar(7): Close-up of underside of leaves of squaw-weed (Senecio ampullaceus ... Creek Park. College Station, Texas, March 20, 2023
Similar(8): Tissues (in Melzer) of sunflower white rust fungus Pustula ... on-the-Brazos a day before. Texas, March 7, 2023